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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | peptidylprolyl cis/trans isomerase, NIMA-interacting 1 |
| Gene Symbol: | PIN1 |
| Synonyms: | DOD; UBL5; [KD Validated] Pin1 |
| Gene ID: | 5300 |
| UniProt ID: | Q13526 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human Pin1 (NP_006212.1). |
| Dilution: | WB 1:1000-1:5000 |
| Purification Method: | Affinity purification |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PIN1 monoclonal antibody |
Background
Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.
Images
 | Western blot analysis of lysates from wild type(WT) and Pin1 knockdown (KD) 293T cells, using [KD Validated] Pin1 Rabbit mAb (A22470) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of various lysates, using [KD Validated] Pin1 Rabbit mAb (A22470) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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