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KD-Validated POLR2L Rabbit mAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | RNA polymerase II, I and III subunit L |
| Gene Symbol: | POLR2L |
| Synonyms: | RBP10; RPB10; RPABC5; RPB7.6; hRPB7.6; RPB10beta |
| Gene ID: | 5441 |
| UniProt ID: | P62875 |
| Clone ID: | 1A8S0 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-67 of human POLR2L (NP_066951.1). |
| Dilution: | WB 1:2000-1:8000; IHC 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.13 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-POLR2L monoclonal antibody |
Background
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains four conserved cysteines characteristic of an atypical zinc-binding domain. Like its counterpart in yeast, this subunit may be shared by the other two DNA-directed RNA polymerases.
Images
 | Western blot analysis of various lysates using [KD Validated] POLR2L Rabbit mAb (A25872) at 1:2200 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Western blot analysis of lysates from 293T cells transfected with POLR2L siRNA using [KD Validated] POLR2L Rabbit mAb (A25872) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) .Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] POLR2L Rabbit mAb (A25872) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KD Validated] POLR2L Rabbit mAb (A25872) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] POLR2L Rabbit mAb (A25872) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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