Your shopping cart is empty!
KD-Validated Peroxiredoxin 6 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | peroxiredoxin 6 |
| Gene Symbol: | PRDX6 |
| Synonyms: | PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; LPCAT-5; HEL-S-128m |
| Gene ID: | 9588 |
| UniProt ID: | P30041 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human Peroxiredoxin 6 (NP_004896.1). |
| Dilution: | WB 1:1000-1:6000; IHC 1:1000-1:10000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.21 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PRDX6 monoclonal antibody |
Background
The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Images
 | Western blot analysis of various lysates using [KD Validated] Peroxiredoxin 6 Rabbit mAb (A27289) at 1:12000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from Rat liver using [KD Validated] Peroxiredoxin 6 Rabbit mAb (A27289) at 1:12000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
 | Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 6 knockdown (KD) HeLa cells using [KD Validated] Peroxiredoxin 6 Rabbit mAb (A27289) at 1:12000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] Peroxiredoxin 6 Rabbit mAb (A27289) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using [KD Validated] Peroxiredoxin 6 Rabbit mAb (A27289) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
You may also be interested in:
