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| Reactivity: | Human |
| Applications: | WB, IHC-P,IF/ICC,IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | prominin 1 |
| Gene Symbol: | PROM1 |
| Synonyms: | RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; [KD Validated] PROM1 |
| Gene ID: | 8842 |
| UniProt ID: | O43490 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 179-433 of human PROM1 (NP_006008.1). |
| Dilution: | WB 1:1000-1:5000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.68 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PROM1 polyclonal antibody |
Background
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from HT-29 cells, using [KD Validated] PROM1 Rabbit pAb (A21701) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from wild type(WT) and PROM1 knockdown (KD) HCT116 cells, using [KD Validated] PROM1 Rabbit pAb (A21701) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CD133 Rabbit pAb (A21701) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney using CD133 Rabbit pAb (A21701) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat lung using CD133 Rabbit pAb (A21701) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of C6 cells using [KD Validated] PROM1 Rabbit pAb (A21701) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HT-29 cells using [KD Validated] PROM1 Rabbit pAb (A21701) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of HT-29 cells using 3 μg CD133 antibody (A21701). Western blot was performed from the immunoprecipitate using CD133 antibody (A21701) at a dilution of 1:1000. |
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