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KD-Validated RHOT1/MIRO1 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ras homolog family member T1 |
| Gene Symbol: | RHOT1 |
| Synonyms: | ARHT1; MIRO1; MIRO-1; [KO/KD Validated] RHOT1/MIRO1 |
| Gene ID: | 55288 |
| UniProt ID: | Q8IXI2 |
| Clone ID: | 6B8R1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 310-590 of human RHOT1/MIRO1 (NP_060777.3). |
| Dilution: | WB 1:1000-1:2000; IHC 1:1000-1:5000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.237 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-RHOT1 monoclonal antibody |
Background
Predicted to enable GTP binding activity and GTPase activity. Involved in cellular homeostasis; mitochondrial outer membrane permeabilization; and mitochondrion transport along microtubule. Is integral component of mitochondrial outer membrane.
Images
 | Western blot analysis of various lysates, using [KD Validated] RHOT1/MIRO1 Rabbit mAb (A22469) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of lysates from wild type(WT) and RHOT1/MIRO1 knockdown (KD) 293T cells, using [KD Validated] RHOT1/MIRO1 Rabbit mAb (A22469) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney using [KO Validated] RHOT1/MIRO1 Rabbit mAb (A22469) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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