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| Reactivity: | Human |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | solute carrier family 16 member 1 |
| Gene Symbol: | SLC16A1 |
| Synonyms: | MCT; HHF7; MCT1; MCT1D |
| Gene ID: | 6566 |
| UniProt ID: | P53985 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-245 of human MCT1 (NP_003042.3). |
| Dilution: | WB 1:2000-1:8000; IHC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1.38 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SLC16A1 monoclonal antibody |
Background
The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and MCT1 knockdown (KD) 293T cells using [KD Validated] MCT1 Rabbit mAb (A27270) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] MCT1 Rabbit mAb (A27270) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] MCT1 Rabbit mAb (A27270) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] MCT1 Rabbit mAb (A27270) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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