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| Reactivity: | Human |
| Applications: | WB, IHC-P, ELISA, ChIP |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | transcription factor EB |
| Gene Symbol: | TFEB |
| Synonyms: | TCFEB; BHLHE35; ALPHATFEB |
| Gene ID: | 7942 |
| UniProt ID: | P19484 |
| Clone ID: | 4N3Z3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-173 of human TFEB (NP_009093.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.94 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TFEB monoclonal antibody |
Background
Enables DNA-binding transcription factor activity; enzyme binding activity; and transcription cis-regulatory region binding activity. Involved in several processes, including cellular response to amino acid starvation; lysosome localization; and positive regulation of autophagy. Located in cytosol; lysosomal membrane; and nucleoplasm.
Images
 | Western blot analysis of lysates from RAW 264.7 cells using [KD Validated] TFEB Rabbit mAb (A25981) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
 | Western blot analysis of lysates from wild type (WT) and TFEB knockdown (KD) 293T cells using [KD Validated] TFEB Rabbit mAb (A25981) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KD Validated] TFEB Rabbit mAb (A25981) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] TFEB Rabbit mAb (A25981) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from Raji cells treated by Torin-1 (250nM, 5h), using 5 μg of [KD Validated] TFEB Rabbit mAb (A25981) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci. |
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