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KD-Validated TOLLIP Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | toll interacting protein |
| Gene Symbol: | TOLLIP |
| Synonyms: | TOLLIP; IL-1RAcPIP; toll-interacting protein |
| Gene ID: | 54472 |
| UniProt ID: | Q9H0E2 |
| Clone ID: | 6L9Z2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human TOLLIP. (NP_061882.2). |
| Dilution: | WB 1:8000-1:32000; IHC 1:100-1:400; IF/IC 1:100-1:400 |
| Purification Method: | Affinity purification |
| Concentration: | 1.74 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TOLLIP monoclonal antibody |
Background
This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of various lysates, using [KD Validated] TOLLIP Rabbit mAb (A23780) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from wild type(WT) and TOLLIP knockdown (KD) 293T cells, using [KD Validated] TOLLIP Rabbit mAb (A23780) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse testis using [KD Validated] TOLLIP Rabbit mAb (A23780) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat testis using [KD Validated] TOLLIP Rabbit mAb (A23780) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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