Your shopping cart is empty!
KD-Validated TRIM25 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | tripartite motif containing 25 |
| Gene Symbol: | TRIM25 |
| Synonyms: | EFP; Z147; RNF147; ZNF147 |
| Gene ID: | 7706 |
| UniProt ID: | Q14258 |
| Clone ID: | 7O6S6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-379 of human TRIM25 (NP_005073.2). |
| Dilution: | WB 1:100-1:500; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.35 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TRIM25 monoclonal antibody |
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity.
Images
 | Western blot analysis of lysates from wild type (WT) and TRIM25 knockdown (KD) 293T cells using [KD Validated] TRIM25 Rabbit mAb (A25846) at 1:4700 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] TRIM25 Rabbit mAb (A25846) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Confocal imaging of HeLa cells using [KD Validated] TRIM25 Rabbit mAb (A25846, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Immunoprecipitation of TRIM25 from 300 µg extracts of 293T cells was performed using 3 µg of [KD Validated] TRIM25 Rabbit mAb (A25846). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KD Validated] TRIM25 Rabbit mAb (A25846) at a dilution of 1:5000. |
You may also be interested in:
