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KD-Validated YTHDC2 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IF/ICC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | YTH N6-methyladenosine RNA binding protein C2 |
| Gene Symbol: | YTHDC2 |
| Synonyms: | CAHL; hYTHDC2; YTHDC2 |
| Gene ID: | 64848 |
| UniProt ID: | Q9H6S0 |
| Immunogen: | Recombinant Protein corresponding to a sequence within amino acids 1286-1430 of human YTHDC2(NP_073739.3). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.41 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-YTHDC2 polyclonal antibody |
Background
This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line.
Images
 | Western blot analysis of various lysates using [KD Validated] YTHDC2 Rabbit pAb (A24219) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type (WT) and YTHDC2 knockdown (KD) HeLa cells using [KD Validated] YTHDC2 Rabbit pAb (A24219) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of paraffin-embedded Mouse testis using [KD Validated] YTHDC2 Rabbit pAb(A24219) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of paraffin-embedded Rat testis using [KD Validated] YTHDC2 Rabbit pAb(A24219) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation of YTHDC2 in 200 µg extracts from 293T cells using 0.5 µg [KD Validated] YTHDC2 Rabbit pAb (A24219). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit pAb (A24219) at 1:1000 dilution. |
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