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KD-Validated ZNF143 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | zinc finger protein 143 |
| Gene Symbol: | ZNF143 |
| Synonyms: | SBF; STAF; pHZ-1; [KD Validated] ZNF143 |
| Gene ID: | 7702 |
| UniProt ID: | P52747 |
| Clone ID: | 4K1I1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 592-638 of human ZNF143 (NP_003433.3). |
| Dilution: | WB 1:1000-1:5000; IHC 1:50-1:200; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 2.32 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ZNF143 monoclonal antibody |
Background
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in positive regulation of snRNA transcription by RNA polymerase II. Predicted to be located in nucleoplasm.
Images
 | Western blot analysis of various lysates, using [KD Validated] ZNF143 Rabbit mAb (A24947) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from wild type (WT) and ZNF143 knockdown (KD) 293F cells using [KD Validated] ZNF143 Rabbit mAb (A24947) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] ZNF143 Rabbit mAb (A24947) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human skin tissue using [KD Validated] ZNF143 Rabbit mAb (A24947) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] ZNF143 Rabbit mAb (A24947) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using [KD Validated] ZNF143 Rabbit mAb (A24947) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using [KD Validated] ZNF143 Rabbit mAb (A24947) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KD Validated] ZNF143 Rabbit mAb (A24947) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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