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| Reactivity: | Human |
| Applications: | WB, IHC-P, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | anaphase promoting complex subunit 5 |
| Gene Symbol: | ANAPC5 |
| Synonyms: | APC5; C5 |
| Gene ID: | 51433 |
| UniProt ID: | Q9UJX4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2). |
| Dilution: | WB 1:2000-1:6000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.21 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ANAPC5 polyclonal antibody |
Background
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
Images
 | Western blot analysis of various lysates using [KO Validated] ANAPC5 Rabbit pAb (A7109) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from wild type (WT) and ANAPC5 knockout (KO) 293T cells, using [KO Validated] ANAPC5 Rabbit pAb (A7109) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney using [KO Validated] ANAPC5 Rabbit pAb (A7109) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] ANAPC5 Rabbit pAb (A7109) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg ANAPC5 antibody (A7109). Western blot was performed from the immunoprecipitate using ANAPC5 antibody (A7109) at a dilution of 1:1000. |
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