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| Reactivity: | Human |
| Applications: | WB, IF/ICC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ATP synthase inhibitory factor subunit 1 |
| Gene Symbol: | ATP5IF1 |
| Synonyms: | IP; ATPI; ATPIP; ATPIF1; F1 |
| Gene ID: | 93974 |
| UniProt ID: | Q9UII2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1). |
| Dilution: | WB 1:1000-1:6000; IHC 1:1000-1:4000; IF/IC 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.11 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ATP5IF1 polyclonal antibody |
Background
Enables several functions, including ATPase binding activity; angiostatin binding activity; and mitochondrial proton-transporting ATP synthase complex binding activity. Involved in several processes, including mitochondrial depolarization; negative regulation of ATPase activity; and regulation of protein targeting to mitochondrion. Located in cell surface and mitochondrion.
Images
 | Western blot analysis of lysates from wild type(WT) and ATPIF1 knockout (KO) 293T(KO) cells, using [KO Validated] ATPIF1 Rabbit pAb (A5099) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U2OS cells using [KO Validated] ATPIF1 Rabbit pAb (A5099) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg [KO Validated] ATPIF1 Rabbit pAb (A5099). Western blot was performed from the immunoprecipitate using [KO Validated] ATPIF1 Rabbit pAb (A5099) at a dilition of 1:3000. |
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