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KO-Validated CDK2AP1 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | cyclin dependent kinase 2 associated protein 1 |
| Gene Symbol: | CDK2AP1 |
| Synonyms: | DOC1; ST19; DORC1; doc-1; p12DOC-1; P1 |
| Gene ID: | 8099 |
| UniProt ID: | O14519 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human CDK2AP1 (NP_004633.1). |
| Dilution: | WB 1:1000-1:5000 |
| Purification Method: | Affinity purification |
| Concentration: | 3.92 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CDK2AP1 polyclonal antibody |
Background
The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Images
 | Western blot analysis of lysates from wild type (WT) and CDK2AP1 knockout (KO) 293T cells, using [KO Validated] CDK2AP1 Rabbit pAb (A19985) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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