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KO-Validated Claudin 1 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | claudin 1 |
| Gene Symbol: | CLDN1 |
| Synonyms: | CLD1; SEMP1; ILVASC; 1 |
| Gene ID: | 9076 |
| UniProt ID: | O95832 |
| Clone ID: | 9C3P1 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 112-211 of human Claudin 1 (NP_066924.1). |
| Dilution: | WB 1:12000-1:72000; IHC 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.69 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CLDN1 monoclonal antibody |
Background
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
Images
 | Western blot analysis of various lysates using [KO Validated] Claudin 1 Rabbit mAb (A21971) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of various lysates using [KO Validated] Claudin 1 Rabbit mAb (A21971) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type(WT) and Claudin 1 knockdown (KD) Hep G2 cells, using [KO Validated] Claudin 1 Rabbit mAb (A21971) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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