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KO-Validated PHD2/EGLN1 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | egl-9 family hypoxia inducible factor 1 |
| Gene Symbol: | EGLN1 |
| Synonyms: | HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2; N1 |
| Gene ID: | 54583 |
| UniProt ID: | Q9GZT9 |
| Clone ID: | 0K3D3 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD2/EGLN1 (NP_071334.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.20 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-EGLN1 monoclonal antibody |
Background
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
Images
 | Western blot analysis of lysates from wild type (WT) and PHD2/EGLN1 knockout (KO) 293T(KO) cells, using [KO Validated] PHD2/EGLN1 Rabbit mAb (A21252) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 8s. |
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