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KO-Validated EIF2AK1 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | eukaryotic translation initiation factor 2 alpha kinase 1 |
| Gene Symbol: | EIF2AK1 |
| Synonyms: | HCR; HRI; hHRI; LEMSPAD; K1 |
| Gene ID: | 27102 |
| UniProt ID: | Q9BQI3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 371-630 of human EIF2AK1 (NP_055228.2). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.84 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-EIF2AK1 polyclonal antibody |
Background
The protein encoded by the gene EIF2AK1 acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of various lysates, using [KO Validated] EIF2AK1 Rabbit pAb (A3415) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Western blot analysis of lysates from wild type(WT) and EIF2AK1 Rabbit pAb knockout (KO) 293T cells, using [KO Validated] EIF2AK1 Rabbit pAb (A3415) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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