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| Reactivity: | Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | endothelial differentiation-related factor 1 |
| Gene Symbol: | Edf1 |
| Synonyms: | MBF1; 0610008L11Rik; f1 |
| Gene ID: | 59022 |
| UniProt ID: | Q9JMG1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of mouse Edf1 (NP_067494.1). |
| Dilution: | WB 1:1000-1:4000; IF/IC 1:200-1:400 |
| Purification Method: | Affinity purification |
| Concentration: | 0.79mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Edf1 polyclonal antibody |
Background
Predicted to enable TFIID-class transcription factor complex binding activity and transcription coactivator activity. Predicted to be involved in positive regulation of DNA binding activity. Predicted to act upstream of or within cell differentiation. Predicted to be located in cytosol; nucleolus; and nucleoplasm. Predicted to be active in nucleus. Is expressed in several structures, including central nervous system; genitourinary system; heart; liver; and retina. Orthologous to human EDF1 (endothelial differentiation related factor 1).
Images
 | Western blot analysis of lysates from wild type (WT) and Edf1 knockout (KO) NIH/3T3 cells, using [KO Validated] Edf1 Rabbit pAb (A20274) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Western blot analysis of lysates from HT-29 cells, using [KO Validated] Edf1 Rabbit pAb (A20274) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
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