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KO-Validated Flotillin 1 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | Flotillin 1 |
| Gene Symbol: | FLOT1 |
| Synonyms: | FLOT1; 1 |
| Gene ID: | 10211 |
| UniProt ID: | O75955 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 155-427 of human Flotillin 1 (NP_005794.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.76 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-FLOT1 polyclonal antibody |
Background
This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from wild type (WT) and Flotillin 1 knockout (KO) 293T cells, using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of various lysates, using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat heart using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Flotillin 1 antibody (A6220). Western blot was performed from the immunoprecipitate using Flotillin 1 antibody (A6220) at a dilution of 1:1000. |
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