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KO-Validated C21orf33 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | glutamine amidotransferase class 1 domain containing 3 |
| Gene Symbol: | GATD3 |
| Synonyms: | ES1; HES1; KNPH; KNPI; GT335; GATD3A; C21orf33; 33 |
| Gene ID: | 8209 |
| UniProt ID: | P30042 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 42-268 of human C21orf33 (NP_004640.4). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 1.92 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GATD3 polyclonal antibody |
Background
This gene encodes a potential mitochondrial protein that is a member of the DJ-1/PfpI gene family. This protein is overexpressed in fetal Down syndrome brain. Alternate splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from wild type(WT) and C21orf33 knockout (KO) 293T(KO) cells, using [KO Validated] C21orf33 Rabbit pAb (A6429) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U2OS cells using [KO Validated] C21orf33 Rabbit pAb (A6429).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. |
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