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| Reactivity: | Human |
| Applications: | WB, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | glutathione S-transferase kappa 1 |
| Gene Symbol: | GSTK1 |
| Synonyms: | GST; GST13; hGSTK1; GSTK1-1; GST13-13; GST 13-13; GSTK1 |
| Gene ID: | 373156 |
| UniProt ID: | Q9Y2Q3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human GSTK1 (NP_057001.1). |
| Purification Method: | Affinity purification |
| Concentration: | 0.47 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GSTK1 polyclonal antibody |
Background
This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from wild type (WT) and GSTK1 knockout (KO) 293T cells using [KO Validated] GSTK1 Rabbit pAb (A21473) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of various lysates using [KO Validated] GSTK1 Rabbit pAb (A21473) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of Hela cells using [KO Validated] GSTK1 Rabbit pAb (A21473) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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