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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | glutathione S-transferase omega 1 |
| Gene Symbol: | GSTO1 |
| Synonyms: | P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21; O1 |
| Gene ID: | 9446 |
| UniProt ID: | P78417 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human GSTO1 (NP_004823.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.72 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GSTO1 polyclonal antibody |
Background
The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and GSTO1 knockout (KO) 293T cells, using [KO Validated] GSTO1 Rabbit pAb (A19892) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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