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KO-Validated HIF1AN/FIH1 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | hypoxia inducible factor 1 subunit alpha inhibitor |
| Gene Symbol: | HIF1AN |
| Synonyms: | FIH1; H1 |
| Gene ID: | 55662 |
| UniProt ID: | Q9NWT6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN/FIH1 (NP_060372.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.57 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HIF1AN polyclonal antibody |
Background
Enables several functions, including 2-oxoglutarate-dependent dioxygenase activity; NF-kappaB binding activity; and transition metal ion binding activity. Involved in several processes, including negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter in response to hypoxia; and protein hydroxylation. Located in cytosol; nucleoplasm; and perinuclear region of cytoplasm. Colocalizes with nucleus.
Images
 | Western blot analysis of various lysates using [KO Validated] HIF1AN/FIH1 Rabbit pAb (A5466) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type (WT) and HIF1AN/FIH1 knockout (KO) 293T cells, using [KO Validated] HIF1AN/FIH1 Rabbit pAb (A5466) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using [KO Validated] HIF1AN/FIH1 Rabbit pAb (A5466) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] HIF1AN/FIH1 Rabbit pAb (A5466) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KO Validated] HIF1AN/FIH1 Rabbit pAb (A5466) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of L929 cells using [KO Validated] HIF1AN/FIH1 Rabbit pAb (A5466) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg HIF1AN/FIH1 antibody (A5466). Western blot was performed from the immunoprecipitate using HIF1AN/FIH1 antibody (A5466) at a dilution of 1:1000. |
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