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| Reactivity: | Human |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | inhibitor of nuclear factor kappa B kinase regulatory subunit gamma |
| Gene Symbol: | IKBKG |
| Synonyms: | IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; SAIDX; AMCBX1; EDAID1; IKKAP1; ZC2HC9; IKK-gamma; Kγ |
| Gene ID: | 8517 |
| UniProt ID: | Q9Y6K9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human IKKγ (NP_003630.1). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:1000-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.30 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-IKBKG polyclonal antibody |
Background
This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
Images
 | Western blot analysis of various lysates using [KO Validated] IKKγ Rabbit pAb (A12536) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Western blot analysis of lysates from wild type (WT) and IKKγ knockout (KO) 293T cells, using [KO Validated] IKKγ Rabbit pAb (A12536) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunoprecipitation analysis of 200 μg extracts of A549 cells using 3 μg IKKγ antibody (A12536). Western blot was performed from the immunoprecipitate using IKKγ antibody (A12536) at a dilution of 1:1000. |
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