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KO-Validated Cytokeratin 20 (KRT20) Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | keratin 20 |
| Gene Symbol: | KRT20 |
| Synonyms: | K20; CD20; CK20; CK-20; KRT21; 0) |
| Gene ID: | 54474 |
| UniProt ID: | P35900 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 245-424 of human Cytokeratin 20 (Cytokeratin 20 (KRT20)) (NP_061883.1). |
| Dilution: | WB 1:1000-1:4000; IHC 1:100-1:1000; IF/IC 1:100-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.62 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-KRT20 polyclonal antibody |
Background
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
Images
 | Western blot analysis of lysates from wild type (WT) and Cytokeratin 20 (Cytokeratin 20 (KRT20)) knockout (KO) HeLa cells, using [KO Validated] Cytokeratin 20 (KRT20) Rabbit pAb (A17997) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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