Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | mutS homolog 2 |
| Gene Symbol: | MSH2 |
| Synonyms: | FCC1; COCA1; HNPCC; LCFS2; MSH-2; hMSH2; HNPCC1; LYNCH1; MMRCS2; H2 |
| Gene ID: | 4436 |
| UniProt ID: | P43246 |
| Clone ID: | 3N6Z3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 330-583 of human MSH2 (NP_000242.1). |
| Dilution: | WB 1:2000-1:20000; IHC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 0.07 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MSH2 monoclonal antibody |
Background
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type(WT) and MSH2 knockout (KO) HeLa cells, using [KO Validated] MSH2 Rabbit mAb (A22177) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of various lysates, using [KO Validated] MSH2 Rabbit mAb (A22177) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] MSH2 Rabbit mAb (A22177) at dilution of 1:400 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] MSH2 Rabbit mAb (A22177) at dilution of 1:400 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining. |
You may also be interested in:
