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KO-Validated NBS1/NBN Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | nibrin |
| Gene Symbol: | NBN |
| Synonyms: | ATV; NBS; P95; NBS1; AT-V1; AT-V2; BN |
| Gene ID: | 4683 |
| UniProt ID: | O60934 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 520-754 of human NBS1/NBN (NP_002476.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.89 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NBN polyclonal antibody |
Background
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
Images
 | Western blot analysis of lysates from wild type (WT) and NBS1/NBS1/NBN knockout (KO) 293T cells, using [KO Validated] NBS1/NBN Rabbit pAb (A0783) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] NBS1/NBS1/NBN Rabbit pAb (A0783) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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