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| Reactivity: | Human |
| Applications: | WB, IP, ELISA, ChIP |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NFE2 like bZIP transcription factor 2 |
| Gene Symbol: | NFE2L2 |
| Synonyms: | NRF2; HEBP1; Nrf-2; IMDDHH; F2 |
| Gene ID: | 4780 |
| UniProt ID: | Q16236 |
| Clone ID: | 9Y2Z6 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 505-605 of human NRF2 (NP_006155.2). |
| Dilution: | WB 1:1000-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NFE2L2 monoclonal antibody |
Background
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
Images
 | Chromatin immunoprecipitation was performed with cross-linked chromatin from HepG2 cells, using Nrf2 Rabbit mAb (A3577) and rabbit IgG (AC042). The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input. |
 | Western blot analysis of extracts from wild type(WT) and NRF2 knockout (KO) HeLa cells, using [KO Validated] NRF2 Rabbit mAb (A3577) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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