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KO-Validated PD-1/CD279 Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | programmed cell death 1 |
| Gene Symbol: | PDCD1 |
| Synonyms: | PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; 79 |
| Gene ID: | 5133 |
| UniProt ID: | Q15116 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-170 of human PD-1/CD279 (NP_005009.2). |
| Dilution: | WB 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 1.93 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PDCD1 polyclonal antibody |
Background
Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity.
Images
 | Western blot analysis of various lysates using [KO Validated] PD-1/CD279 Rabbit pAb (A5584) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from Raji cells using PD-1/CD279 Rabbit pAb (A5584) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Western blot analysis of lysates from wild type (WT) and PD-1/CD279 knockout (KO) 293T cells, using [KO Validated] PD-1/CD279 Rabbit pAb (A5584) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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