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KO-Validated DNA Polymerase beta Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | DNA polymerase beta |
| Gene Symbol: | POLB |
| Synonyms: | POLB; ta |
| Gene ID: | 5423 |
| UniProt ID: | P06746 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human DNA Polymerase beta (NP_002681.1). |
| Dilution: | WB 1:200-1:1000; IHC 1:50-1:200; IF/IC 1:20-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.22 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-POLB polyclonal antibody |
Background
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.
Images
 | Western blot analysis of various lysates using [KO Validated] DNA Polymerase beta Rabbit pAb (A1681) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Western blot analysis of lysates from wild type (WT) and DNA Polymerase beta knockout (KO) HeLa cells, using [KO Validated] DNA Polymerase beta Rabbit pAb (A1681) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human stomach using [KO Validated] DNA Polymerase beta Rabbit pAb (A1681) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] DNA Polymerase beta Rabbit pAb (A1681) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of U2OS cells using [KO Validated] DNA Polymerase beta Rabbit pAb (A1681). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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