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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | protein arginine methyltransferase 8 |
| Gene Symbol: | PRMT8 |
| Synonyms: | HRMT1L3; HRMT1L4; T8 |
| Gene ID: | 56341 |
| UniProt ID: | Q9NR22 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human PRMT8 (NP_062828.3). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.85 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PRMT8 polyclonal antibody |
Background
Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).
Images
 | Western blot analysis of lysates from wild type (WT) and PRMT8 knockout (KO) 293T cells, using [KO Validated] PRMT8 Rabbit pAb (A18440) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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