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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | RAB1B, member RAS oncogene family |
| Gene Symbol: | RAB1B |
| Synonyms: | RAB1B |
| Gene ID: | 81876 |
| UniProt ID: | Q9H0U4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 7-201 of human RAB1B (NP_112243.1). |
| Dilution: | WB 1:13000-1:78000; IHC 1:600-1:3000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.44 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-RAB1B polyclonal antibody |
Background
Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).
Images
 | Western blot analysis of lysates from wild type (WT) and RAB1B knockout (KO) HeLa cells using [KO Validated] RAB1B Rabbit pAb RAB1B Rabbit pAb (A7514) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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