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KO-Validated Rad23A Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | RAD23 homolog A, nucleotide excision repair protein |
| Gene Symbol: | RAD23A |
| Synonyms: | HR23A; HHR23A; 3A |
| Gene ID: | 5886 |
| UniProt ID: | P54725 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human Rad23A (NP_005044.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.82 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-RAD23A polyclonal antibody |
Background
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms.
Images
 | Western blot analysis of lysates from wild type (WT) and Rad23A knockout (KO) 293T cells, using [KO Validated] Rad23A Rabbit pAb (A19884) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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