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KO-Validated NF-kB p65/RelA Rabbit mAb (20 μl)
| Reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC, IF/IC,IP, ELISA, ChIP |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | RELA proto-oncogene, NF-kB subunit |
| Gene Symbol: | RELA |
| Synonyms: | p65; CMCU; NFKB3; AIF3BL3; lA |
| Gene ID: | 5970 |
| UniProt ID: | Q04206 |
| Clone ID: | 6D6R8 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-kB p65/RelA (NP_068810.3). |
| Dilution: | WB 1:10000-1:60000; IHC 1:200-1:2000; IF/IC 1:100-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-RELA monoclonal antibody |
Background
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of various lysates using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type(WT) and NF-kB p65/RelA knockout (KO) HeLa cells, using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Confocal imaging of HeLa cells using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331, dilution 1:100) (Red). The cells were counterstained with MonoMethyl-Histone H3-K4 Rabbit mAb (A22078, dilution 1:300) (Green). DAPI was used for nuclear staining (blue). Objective: 60x. |
 | Immunoprecipitation of [KO Validated] NF-kB p65/RelA Rabbit mAb from 500 µg extracts of HeLa cells was performed using 2 µg of [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at a dilution of 1:10000. |
 | Chromatin immunoprecipitation analysis of extracts of HT-1080 cells, HT-1080 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes, using NF-kB p65/RelA antibody (A22331) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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