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| Reactivity: | Human |
| Applications: | WB, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | SET domain containing 7, histone lysine methyltransferase |
| Gene Symbol: | SETD7 |
| Synonyms: | KMT7; SET7; SET9; SET7/9; D7 |
| Gene ID: | 80854 |
| UniProt ID: | Q8WTS6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_085151.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.48 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SETD7 polyclonal antibody |
Background
Enables histone-lysine N-methyltransferase activity and p53 binding activity. Involved in peptidyl-lysine dimethylation and peptidyl-lysine monomethylation. Acts upstream of or within cellular response to DNA damage stimulus and heterochromatin organization. Located in chromosome and nucleolus.
Images
 | Western blot analysis of lysates from wild type (WT) and SETD7 knockout (KO) 293T cells, using [KO Validated] SETD7 Rabbit pAb (A9985) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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