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KO-Validated SLC25A22 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | solute carrier family 25 member 22 |
| Gene Symbol: | SLC25A22 |
| Synonyms: | GC1; DEE3; GC-1; EIEE3; NET44; 22 |
| Gene ID: | 79751 |
| UniProt ID: | Q9H936 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 128-188 of human SLC25A22 (NP_078974.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 1.71 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SLC25A22 polyclonal antibody |
Background
This gene encodes a mitochondrial glutamate carrier. Mutations in this gene are associated with early infantile epileptic encephalopathy. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Images
 | Western blot analysis of various lysates using [KO Validated] SLC25A22 Rabbit pAb (A17772) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type (WT) and SLC25A22 knockout (KO) 293T cells, using [KO Validated] SLC25A22 Rabbit pAb (A17772) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using [KO Validated] SLC25A22 Rabbit pAb (A17772) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using [KO Validated] SLC25A22 Rabbit pAb (A17772) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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