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KO-Validated SMARCC1/BAF155 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | SWI/SNF related BAF chromatin remodeling complex subunit C1 |
| Gene Symbol: | SMARCC1 |
| Synonyms: | HYC5; Rsc8; SRG3; SWI3; BAF155; CRACC1; 55 |
| Gene ID: | 6599 |
| UniProt ID: | Q92922 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1/BAF155 (NP_003065.3). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.06 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SMARCC1 polyclonal antibody |
Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Images
 | Western blot analysis of lysates from wild type (WT) and SMARCC1/BAF155 knockout (KO) 293T cells, using [KO Validated] SMARCC1/BAF155 Rabbit pAb (A6128) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg SMARCC1/BAF155 antibody (A6128). Western blot was performed from the immunoprecipitate using SMARCC1/BAF155 antibody (A6128) at a dilution of 1:1000. |
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