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KO-Validated BAF57/SMARCE1 Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | SWI/SNF related BAF chromatin remodeling complex subunit E1 |
| Gene Symbol: | SMARCE1 |
| Synonyms: | CSS5; BAF57; E1 |
| Gene ID: | 6605 |
| UniProt ID: | Q969G3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human BAF57/BAF57/SMARCE1 (NP_003070.3). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.19 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SMARCE1 polyclonal antibody |
Background
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
Images
 | Western blot analysis of various lysates using [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from wild type (WT) and BAF57/SMARCE1 knockout (KO) 293T cells, using [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Immunofluorescence analysis of HeLa cells using [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. |
 | Immunoprecipitation analysis of 150 μg extracts of Jurkat cells using 3 μg BAF57/BAF57/SMARCE1 antibody (A13353). Western blot was performed from the immunoprecipitate using BAF57/BAF57/SMARCE1 antibody (A13353) at a dilution of 1:1000. |
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