Reactivity: | Human |
Applications: | WB, IHC, IF/IC, IP, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | staphylococcal nuclease and tudor domain containing 1 |
Gene Symbol: | SND1 |
Synonyms: | p100; TDRD11; Tudor-SN; D1 |
Gene ID: | 27044 |
UniProt ID: | Q7KZF4 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-285 of human SND1 (NP_055205.2). |
Dilution: | WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
Purification Method: | Affinity purification |
Concentration: | 2.19 mg/mL |
Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-SND1 polyclonal antibody |
Background
The gene SND1 encodes a transcriptional co-activator that interacts with the acidic domain of Epstein-Barr virus nuclear antigen 2 (EBNA 2), a transcriptional activator that is required for B-lymphocyte transformation. Other transcription factors that interact with this protein are signal transducers and activators of transcription, STATs. This protein is also thought to be essential for normal cell growth. A similar protein in mammals and other organisms is a component of the RNA-induced silencing complex (RISC).
Images
![]() | Western blot analysis of various lysates using [KO Validated] SND1 Rabbit pAb (A5874) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![]() | Western blot analysis of various lysates using [KO Validated] SND1 Rabbit pAb (A5874) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![]() | Western blot analysis of various lysates, using SND1 Rabbit pAb (A5874) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
![]() | Western blot analysis of lysates from wild type (WT) and SND1 knockout (KO) HeLa cells, using [KO Validated] SND1 Rabbit pAb (A5874) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![]() | Western blot analysis of lysates from wild type (WT) and SND1 knockout (KO) HeLa cells, using [KO Validated] SND1 Rabbit pAb (A5874) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![]() | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Rat colon using [KO Validated] SND1 Rabbit pAb (A5874) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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