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| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | succinate-CoA ligase GDP-forming subunit beta |
| Gene Symbol: | SUCLG2 |
| Synonyms: | G-SCS; GBETA; GTPSCS; G2 |
| Gene ID: | 8801 |
| UniProt ID: | Q96I99 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-300 of human SUCLG2 (NP_003839.2). |
| Dilution: | WB 1:2000-1:8000; IHC 1:300-1:3000; IF/IC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 5.94 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SUCLG2 polyclonal antibody |
Background
This gene encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 5 and 12.
Images
 | Western blot analysis of lysates from wild type(WT) and SUCLG2 knockout (KO) 293T(KO) cells, using [KO Validated] SUCLG2 Rabbit pAb (A8976) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] SUCLG2 Rabbit pAb (A8976) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] SUCLG2 Rabbit pAb (A8976) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of C6 cells using [KO Validated] SUCLG2 Rabbit pAb (A8976) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using [KO Validated] SUCLG2 Rabbit pAb (A8976) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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