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| Reactivity: | Human |
| Applications: | WB, IF/ICC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | SUZ12 polycomb repressive complex 2 subunit |
| Gene Symbol: | SUZ12 |
| Synonyms: | CHET9; IMMAS; JJAZ1; 12 |
| Gene ID: | 23512 |
| UniProt ID: | Q15022 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 0.78 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SUZ12 polyclonal antibody |
Background
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by the gene SUZ12 contains a zinc finger domain in the C terminus of the coding region.
Images
 | Western blot analysis of various lysates using [KO Validated] SUZ12 Rabbit pAb (A7786) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
_KO-WB_01.jpg?t=1702605770) | Western blot analysis of lysates from wild type (WT) and SUZ12 knockout (KO) HeLa cells, using [KO Validated] SUZ12 Rabbit pAb (A7786) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using [KO Validated] SUZ12 Rabbit pAb (A7786) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg SUZ12 antibody (A7786). Western blot was performed from the immunoprecipitate using SUZ12 antibody (A7786) at a dilution of 1:500. |
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