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| Reactivity: | Human |
| Applications: | WB, IF/ICC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ubiquitin conjugating enzyme E2 O |
| Gene Symbol: | UBE2O |
| Synonyms: | E2-230K; 2O |
| Gene ID: | 63893 |
| UniProt ID: | Q9C0C9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.05 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-UBE2O polyclonal antibody |
Background
Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway; protein ubiquitination; and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.
Images
 | Western blot analysis of various lysates using [KO Validated] UBE2O Rabbit pAb (A10036) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from wild type (WT) and UBE2O knockout (KO) HeLa cells, using [KO Validated] UBE2O Rabbit pAb (A10036) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of HeLa cells using [KO Validated] UBE2O Rabbit pAb (A10036) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of A-431 cells using 3 μg UBE2O antibody (A10036). Western blot was performed from the immunoprecipitate using UBE2O antibody (A10036) at a dilution of 1:1000. |
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