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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | vav guanine nucleotide exchange factor 2 |
| Gene Symbol: | VAV2 |
| Synonyms: | VAV-2; V2 |
| Gene ID: | 7410 |
| UniProt ID: | P52735 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 570-700 of human VAV2 (NP_001127870.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.43 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-VAV2 polyclonal antibody |
Background
VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and VAV2 knockout (KO) HeLa cells, using [KO Validated] VAV2 Rabbit pAb (A19883) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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