Your shopping cart is empty!
KO-Validated ZMYND8 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | zinc finger MYND-type containing 8 |
| Gene Symbol: | ZMYND8 |
| Synonyms: | RACK7; PRKCBP1; PRO2893; D8 |
| Gene ID: | 23613 |
| UniProt ID: | Q9ULU4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human ZMYND8 (NP_898868.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.32 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ZMYND8 polyclonal antibody |
Background
The protein encoded by the gene ZMYND8 is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and ZMYND8 knockout (KO) HeLa cells, using [KO Validated] ZMYND8 Rabbit pAb (A21848) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of various lysates using [KO Validated] ZMYND8 Rabbit pAb (A21848) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunoprecipitation analysis of 300 μg extracts of U-87 cells using 3 μg [KO Validated] ZMYND8 Rabbit pAb (A21848). Western blot was performed from the immunoprecipitate using [KO Validated] ZMYND8 Rabbit pAb (A21848) at a dilition of 1:1000. |
You may also be interested in:
