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| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ATG3 rabbit polyclonal antibody |
Gene Name: | Ubiquitin-like-conjugating enzyme ATG3 |
Synonyms: | Autophagy-related protein 3, Protein PC3-96, APG3-like, hApg3, ATG3, APG3, APG3L |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ATG3 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 36 kDa |
Observed MW. | 40 kDa |
Uniprot ID: | Q9CPX6, Q6AZ50 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 500-1: 1000 | muscle |
Background
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Images
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Western blot analysis of ATG3 (GB11839) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
