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Anti-ATP5A Rabbit pAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ATP5A rabbit polyclonal antibody |
Gene Name: | ATP synthase subunit alpha, mitochondrial |
Synonyms: | ATP synthase F1 subunit alpha, Atp5f1a, Atp5a1, Modifier of Min 2 mouse homolog, hATP1, Epididymis secretory sperm binding protein Li 123m, ATP5AL2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ATP5A |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Mitochondrion, Mitochondrion inner membrane |
Predicted MW. | 60 kDa |
Observed MW. | 50 kDa |
Uniprot ID: | Q03265, P15999 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | brain, lung, heart |
IHC/IF | Mouse, Rat | 1: 300-1: 600 | heart, hippocampus, spinal cord, stomach |
Background
ATP5J (ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6) is a multisubunit membrane-bound enzyme complex consisting of an F0 segment embedded in the membrane and an F1 segment attached to the F0. It is also a component of mitochondrial ATP synthase which is required for the interactions of the catalytic and proton-translocating segments. Human ATP5J shares 72% sequence identity with rat ATP5J.
Images
 | Western blot analysis of ATP5A1 (GB112433) at dilution of 1: 500 |
 | Immunofluorescence of paraffin embedded mouse heart using ATP5A1 (GB112433) at dilution of 1: 300 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse hippocampus using ATP5A1 (GB112433) at dilution of 1: 300 (300x lens) |
 | Immunofluorescence of paraffin embedded mouse spinal cord using ATP5A1 (GB112433) at dilution of 1: 300 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse stomach using ATP5A1 (GB112433) at dilution of 1: 300 (450x lens) |
 | Immunofluorescence of paraffin embedded rat heart using ATP5A1 (GB112433) at dilution of 1: 300 (400x lens) |
 | Immunofluorescence of paraffin embedded rat hippocampus using ATP5A1 (GB112433) at dilution of 1: 300 (200x lens) |
 | Immunofluorescence of paraffin embedded rat stomach using ATP5A1 (GB112433) at dilution of 1: 300 (200x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
