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Anti-ATP5H Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ATP5H rabbit polyclonal |
Gene Name: | ATP synthase subunit d, mitochondrial |
Synonyms: | ATPase subunit d, ATP synthase peripheral stalk subunit d, Atp5pd, Atp5h |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ATP5H |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. | 19 kDa |
Observed MW. | 19 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, liver, heart |
IHC | Human, Mouse, Rat | 1: 9000-1: 18000 | cholangiocarcinoma, lung cancer, ovarian cancer, uterine cancer, gastric cancer |
IF | Human, Mouse, Rat | 1: 500-1: 2000 | ovarian cancer, para-cancer liver |
Background
Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.
Images
| Western blot analysis of ATP5H (GB112434) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded human cholangiocarcinoma using ATP5H (GB112434) at dilution of 1: 9000 (400x lens) |
| Immunohistochemistry of paraffin embedded human metrocarcinoma using ATP5H (GB112434) at dilution of 1: 9000 (400x lens) |
| Immunohistochemistry of paraffin embedded human ovarian cancer using ATP5H (GB112434) at dilution of 1: 9000 (400x lens) |
| Immunohistochemistry of paraffin embedded human renal cancer using ATP5H (GB112434) at dilution of 1: 9000 (400x lens) |
| Immunofluorescence of paraffin embedded human liver using ATP5H (GB112434) at dilution of 1: 650 (400x lens) |
 | Immunofluorescence of paraffin embedded human ovarian cancer using ATP5H (GB112434) at dilution of 1: 500 (400x lens) |
 | Immunofluorescence of paraffin embedded human pancreas using ATP5H (GB112434) at dilution of 1: 650 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse liver using ATP5H (GB112434) at dilution of 1: 650 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse ovary using ATP5H (GB112434) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded rat heart using ATP5H (GB112434) at dilution of 1: 650 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
