Reactivity: | M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | ATPase inhibitor, mitochondrial |
Synonyms: | Atpi, Atpif1 |
Immunogen: | Recombinant protein corresponding to Mouse ATP5IF1 |
Uniprot ID: | O35143 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 12 kDa / 14 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 300-1: 800 | kidney |
Background
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase.Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
Images
WB analysis of ATP5IF1(GB115625). |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |