Recombinant Anti-Acidic Cytokeratin Mouse mAb (100 μl)

Recombinant Anti-Acidic Cytokeratin Mouse mAb (100 μl)

Brand: ServiceBio
Cat. #: GB152050-100
Availability: In Stock
$259.00
-+
Reactivity: H,M,R    
Applications: WB    
Host Species: Mouse    
Clonality: Monoclonal    
Gene Name:

Keratin, type I cytoskeletal 20

Synonyms:

Cytokeratin-20 (CK-20), Keratin-20 (K20), Protein IT

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human CK20

Uniprot ID:

P35900

Isotype:

IgG1

Purity:

Affinity purification

Predicted MW. / Observed MW.

48 kDa / 40-64 kDa




Product Usage Information

WB

Human, Mouse, Rat

1: 500-1: 1000

skin




Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.




Images

01 GB152050_Acidic Cytokeratin_重组H229_d1000_WB_WM.png

WB analysis of Acidic Cytokeratin(GB152050).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.




Storage

Storage            Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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