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Anti-Annexin-4/ANXA4 Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Annexin A4 |
Synonyms: | Annexin IV, Annexin-4, Anxa4, Anx4 |
Immunogen: | KLH conjugated Synthetic peptidecorresponding to Mouse Annexin-4 |
Uniprot ID: | P97429 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Secreted, Cell Membrane |
Predicted MW. / Observed MW. | 36 kDa / 36 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, liver |
IHC/IF | Human, Mouse, Rat | 1: 2000-1: 4000 | gastric cancer, ileum, placenta |
Background
The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin A4 belongs to the annexin family of calcium dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane related events along exocytotic and endocytotic pathways.
Images
| Western blot analysis of Annexin-4 (GB121147) at dilution of 1: 1000 Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A549 cell lysate Lane 4: 293T cell lysate Lane 5: Raw264.7 cell lysate Lane 6: Mouse heart tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Rat heart tissue lysate Lane 9: Rat liver tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Annexin-4 (GB121147) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded mouse ileum using Annexin-4 (GB121147) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded rat ileum using Annexin-4 (GB121147) at dilution of 1: 2000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human cervical cancer using Annexin-4 (GB121147) at dilution of 1: 800 |
| Immunofluorescent analysis of paraformaldehyde-fixed human gastric cancer using Annexin-4 (GB121147) at dilution of 1: 800 |
| Immunofluorescent analysis of paraformaldehyde-fixed human placenta using Annexin-4 (GB121147) at dilution of 1: 800 |
| Immunofluorescent analysis of paraformaldehyde-fixed human small intestine using Annexin-4 (GB121147) at dilution of 1: 800 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse small intestine using Annexin-4 (GB121147) at dilution of 1: 800 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse ileum using Annexin-4 (GB121147) at dilution of 1: 800 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat ileum using Annexin-4 (GB121147) at dilution of 1: 800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
